By A. Trevor Willis
Describes the cultivation, tools of id and scientific value of anaerobic micro organism. wide references and listed
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Extra info for Anaerobic Bacteriology. Clinical and Laboratory Practice
If the agar is too hot when the blood is added, denaturation occurs too rapidly and a coarse, curdy precipitate results. The same thing happens in the poured plates if glass dishes are still very hot from sterilization. Heated blood agar is a generally useful medium for the culture of most anaerobes. Cultures of anaerobes may produce partial clearing of the medium due to proteolytic activity, or slight bleaching due to hydrogen peroxide production (see p. 77). BENZIDINE HEATED BLOOD AGAR (Gordon and McLeod, 1940) Proceed as for heated blood agar.
And Allgeier, D. L. (1965). ' Science, 147, 1033 Brewer, J. H. and Allgeier, D. L. (1966). ' Applied Microbiology, 14, 985 Brewer, J. , Allgeier, D. L. and McLaughlin, C. B. (1966). ' Applied Microbiology, 14, 135 Brewer, J. , Heer, A. A. and McLaughlin, C. B. (1955). ' Applied Micro biology, 3, 136 Bridges, A. , Pepper, R. E. and Chandler, V. L. (1952). ' Journal of Bacteriology, 64, 137 Brown, J. H. (1921). ' Journal of experimental Medicine, 33, 677 Brown, J. H. (1922). ' Journal of experimental Medicine, 35,467 References 29 Bryant, M.
After completion, capsules should be carefully inspected for integrity of the envelope and for any pieces of catalyst which may project outside Appendix 27 it. This is most likely to occur if the mesh of the gauze is too coarse. Any projecting pieces must be removed, otherwise there is a great risk of the glowing catalyst igniting the hydrogen in the jar and causing an explosion. Use of Wright's capsule When the anaerobic jar has been loaded and is ready for setting up, the Wright's capsule is heated by bunsen burner until it is red hot, and is then clipped into position on the underside of the lid before sealing the jar.
Anaerobic Bacteriology. Clinical and Laboratory Practice by A. Trevor Willis